Isolation, identification, and characterization of the human airway ligand for the eosinophil and mast cell immunoinhibitory receptor Siglec-8.

ABSTRACT:

Background

The immunoinhibitory receptor Siglec-8 on the surface of human eosinophils and mast cells binds to sialic acid-containing ligands in the local milieu, resulting in eosinophil apoptosis, inhibition of mast cell degranulation, and suppression of inflammation. Siglec-8 ligands were found on postmortem human trachea and bronchi and on upper airways in 2 compartments, cartilage and submucosal glands, but they were surprisingly absent from the epithelium. We hypothesized that Siglec-8 ligands in submucosal glands and ducts are normally transported to the airway mucus layer, which is lost during tissue preparation.

Objective

Our aim was to identify the major Siglec-8 sialoglycan ligand on the mucus layer of human airways.

Methods

Human upper airway mucus layer pro

SUBMITTER: Gonzalez-Gil A 

PROVIDER: S-EPMC7876160 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature