Hypoxia inducible factor-2α importance for migration, proliferation, and self-renewal of trunk neural crest cells.

ABSTRACT:

Background

The neural crest is a transient embryonic stem cell population. Hypoxia inducible factor (HIF)-2α is associated with neural crest stem cell appearance and aggressiveness in tumors. However, little is known about its role in normal neural crest development.

Results

Here, we show that HIF-2α is expressed in trunk neural crest cells of human, murine, and avian embryos. Knockdown as well as overexpression of HIF-2α in vivo causes developmental delays, induces proliferation, and self-renewal capacity of neural crest cells while decreasing the proportion of neural crest cells that migrate ventrally to sympathoadrenal sites. Reflecting the in vivo phenotype, transcriptome changes after loss of HIF-2α reveal enrichment of genes associated with cancer, invasion, epithelial

SUBMITTER: Niklasson CU 

PROVIDER: S-EPMC7891386 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature