An optimized workflow for CRISPR-Cas9 deletion of surface and intracellular factors in primary human T lymphocytes.

ABSTRACT: The appropriate regulation of T lymphocyte functions is key to achieve protective immune responses, while at the same time limiting the risks of tissue damage and chronic inflammation. Deciphering the mechanisms underpinning T cell responses in humans may therefore be beneficial for a range of infectious and chronic diseases. Recently, the development of methods based on CRISPR-Cas9 gene-editing has greatly expanded the available tool-box for the mechanistic studies of primary human T cell responses. While the deletion of a surface protein has become a relatively straightforward task, as long as an antibody for detection is available, the identification and selection of cells lacking an intracellular protein, a non-coding RNA or a protein for which no antibody is available, remain more pro

SUBMITTER: Leoni C 

PROVIDER: S-EPMC7891767 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature