Large-scale discovery of protein interactions at residue resolution using co-evolution calculated from genomic sequences.

ABSTRACT: Increasing numbers of protein interactions have been identified in high-throughput experiments, but only a small proportion have solved structures. Recently, sequence coevolution-based approaches have led to a breakthrough in predicting monomer protein structures and protein interaction interfaces. Here, we address the challenges of large-scale interaction prediction at residue resolution with a fast alignment concatenation method and a probabilistic score for the interaction of residues. Importantly, this method (EVcomplex2) is able to assess the likelihood of a protein interaction, as we show here applied to large-scale experimental datasets where the pairwise interactions are unknown. We predict 504 interactions de novo in the E. coli membrane proteome, including 243 that are newly disc

SUBMITTER: Green AG 

PROVIDER: S-EPMC7925567 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature