Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP.

ABSTRACT: Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than

SUBMITTER: Bokelmann L 

PROVIDER: S-EPMC7935920 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature