Investigation of urinary components in rat model of ketamine-induced bladder fibrosis based on metabolomics.

ABSTRACT:

Background

Ketamine abuse has been linked to the system's damage, presenting with lower urinary tract symptoms (LUTS). While the pathogenesis of ketamine-induced urinary damage is not fully understood, fibrosis is believed to be a potential mechanism. A metabolomic investigation of the urinary metabolites in ketamine abuse was conducted to gain insights into its pathogenesis.

Methods

A rat model of ketamine induced bladder fibrosis was established through tail vein injection of ketamine hydrochloride and control group was established through tail vein injection of the equivalent normal saline. Hematoxylin and eosin (H&E) staining and Masson trichrome staining were performed to evaluated bladder pathology. Urinary components were detected based on a metabolomic approach using

SUBMITTER: Li H 

PROVIDER: S-EPMC7947432 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature