Dataset Information

Compositional and Functional Changes in the Gut Microbiota in Irritable Bowel Syndrome Patients.

ABSTRACT:

Background/aims

This study aimed to characterize the changes in the gut microbiota of irritable bowel syndrome (IBS) patients and to investigate the consequent alterations in bacterial functions.

Methods

We performed 16S rRNA metagenomic sequencing and a phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) analyses using fecal samples from control (n=12) and diarrhea-dominant IBS patients (n=7).

Results

The samples were clustered by the principal coordinates analysis depending on the presence of IBS (p=0.003). In the IBS patients, the abundances of Acidaminococcaceae, Sutterellaceae, and Desulfovibrionaceae were significantly increased, while those of Enterococcaceae, Leuconostocaceae, Clostridiaceae, Peptostreptococcaceae, and Lachnospiraceae were significantly decreased. The PICRUSt results indicated that two orthologues involved in secondary bile acid biosynthesis were significantly decreased in IBS patients. Modules involved in multidrug resistance, lipopolysaccharide biosynthesis, the reductive citrate cycle, and the citrate cycle were significantly increased in the IBS patients. In contrast, modules involved in cationic antimicrobial peptide resistance, and some transport systems were more abundant in controls than in IBS patients.

Conclusions

Changes in the gut microbiota composition in IBS patients lead to alterations in bacterial functions, such as bile acid transformation and the induction of inflammation, which is a known pathophysiological mechanism of IBS.

SUBMITTER: Lee SM

PROVIDER: S-EPMC7960967 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Publications

Compositional and Functional Changes in the Gut Microbiota in Irritable Bowel Syndrome Patients.

Lee Sun Min SM Kim Nayoung N Yoon Hyuk H Kim Yong Sung YS Choi Soo In SI Park Ji Hyun JH Lee Dong Ho DH

Gut and liver 20210301 2

<h4>Background/aims</h4>This study aimed to characterize the changes in the gut microbiota of irritable bowel syndrome (IBS) patients and to investigate the consequent alterations in bacterial functions.<h4>Methods</h4>We performed 16S rRNA metagenomic sequencing and a phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) analyses using fecal samples from control (n=12) and diarrhea-dominant IBS patients (n=7).<h4>Results</h4>The samples were clustered by the ...[more]

PMID: 32457278

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Dataset Information

Compositional and Functional Changes in the Gut Microbiota in Irritable Bowel Syndrome Patients.

Background/aims

Methods

Results

Conclusions

Publications

Compositional and Functional Changes in the Gut Microbiota in Irritable Bowel Syndrome Patients.

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