Broadly reactive human CD4⁺ T cells against Enterobacteriaceae are found in the naive repertoire and are clonally expanded in the memory repertoire.

ABSTRACT: Enterobacteriaceae are a large family of Gram-negative bacteria that includes both commensals and opportunistic pathogens. The latter can cause severe nosocomial infections, with outbreaks of multi-antibiotics resistant strains, thus being a major public health threat. In this study, we report that Enterobacteriaceae-reactive memory Th cells were highly enriched in a CCR6⁺ CXCR3⁺ Th1*/17 cell subset and produced IFN-γ, IL-17A, and IL-22. This T cell subset was severely reduced in septic patients with K. pneumoniae bloodstream infection who also selectively lacked circulating K. pneumonie-reactive T cells. By combining heterologous antigenic stimulation, single cell cloning and TCR Vβ sequencing, we demonstrate that a large fraction of memory Th cell clones was broadly

SUBMITTER: Cassotta A 

PROVIDER: S-EPMC7986685 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature