Reservoir of Fibroblasts Promotes Recovery From Limb Ischemia.

ABSTRACT:

Background

The angiogenic response to ischemia restores perfusion so as to preserve tissue. A role for mesenchymal-to-endothelial transition in the angiogenic response is controversial. This study is to determine if resident fibroblasts contribute to angiogenesis.

Methods

We utilized the murine model of hindlimb ischemia, and in vivo Matrigel plug assay together with lineage tracing studies and single cell RNA-sequencing to examine the transcriptional and functional changes in fibroblasts in response to ischemia.

Results

Lineage tracing using Fsp1-Cre: R26R-EYFP mice revealed the emergence within the ischemic hindlimb of a small subset of YFP⁺ CD144⁺ CD11b^- fibroblasts (E* cells) that expressed endothelial cell (EC) genes. Subcutaneous

SUBMITTER: Meng S 

PROVIDER: S-EPMC7987209 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature