Transfer of Antibiotic Resistance Plasmid from Commensal E. coli Towards Human Intestinal Microbiota in the M-SHIME: Effect of E. coli dosis, Human Individual and Antibiotic Use.

ABSTRACT: Along with (in) direct contact with animals and a contaminated environment, humans are exposed to antibiotic-resistant bacteria by consumption of food. The implications of ingesting antibiotic-resistant commensal bacteria are unknown, as dose-response data on resistance transfer and spreading in our gut is lacking. In this study, transfer of a resistance plasmid (IncF), harbouring several antibiotic resistance genes, from a commensal E. coli strain towards human intestinal microbiota was assessed using a Mucosal Simulator of the Human Intestinal Ecosystem (M-SHIME). More specifically, the effect of the initial E. coli plasmid donor concentration (10⁵ and 10⁷ CFU/meal), antibiotic treatment (cefotaxime) and human individual (n = 6) on plasmid transfer tow

SUBMITTER: Lambrecht E 

PROVIDER: S-EPMC7997361 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature