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Triggering Neurotransmitters Secretion from Single Cells by X-ray Nanobeam Irradiation.

ABSTRACT: The employment of ionizing radiation is a powerful tool in cancer therapy, but beyond targeted effects, many studies have highlighted the relevance of its off-target consequences. An exhaustive understanding of the mechanisms underlying these effects is still missing, and no real-time data about signals released by cells during irradiation are presently available. We employed a synchrotron X-ray nanobeam to perform the first real-time simultaneous measurement of both X-ray irradiation and in vitro neurotransmitter release from individual adrenal phaeochromocytoma (PC12) cells plated over a diamond-based multielectrode array. We have demonstrated that, in specific conditions, X-rays can alter cell activity by promoting dopamine exocytosis, and such an effect is potentially very attractive for a more effective treatment of tumors.

SUBMITTER: Picollo F

PROVIDER: S-EPMC7997629 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Triggering Neurotransmitters Secretion from Single Cells by X-ray Nanobeam Irradiation.

Picollo Federico F Tomagra Giulia G Bonino Valentina V Carabelli Valentina V Mino Lorenzo L Olivero Paolo P Pasquarelli Alberto A Truccato Marco M

Nano letters 20200403 5

The employment of ionizing radiation is a powerful tool in cancer therapy, but beyond targeted effects, many studies have highlighted the relevance of its off-target consequences. An exhaustive understanding of the mechanisms underlying these effects is still missing, and no real-time data about signals released by cells during irradiation are presently available. We employed a synchrotron X-ray nanobeam to perform the first real-time simultaneous measurement of both X-ray irradiation and in vit ...[more]

PMID: 32227961

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