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Ca2+ -regulated cell migration revealed by optogenetically engineered Ca2+ oscillations.


ABSTRACT: The ability of a single Ca2+ ion to play an important role in cell biology is highlighted by the need for cells to form Ca2+ signals in the dimensions of space, time, and amplitude. Thus, spatial and temporal changes in intracellular Ca2+ concentration are important for determining cell fate. Optogenetic technology has been developed to provide more precise and targeted stimulation of cells. Here, U2OS cells overexpressing Ca2+ translocating channelrhodopsin (CatCh) were used to mediate Ca2+ influx through blue light illumination with various parameters, such as intensity, frequency, duty cycle, and duration. We identified that several Ca2+ -dependent transcription factors and certain kinases can be activated by specific Ca2+ waves. Using a wound-healing assay, we found that low-frequency Ca2+ oscillations increased cell migration through the activation of NF-κB. This study explores the regulation of cell migration by Ca2+ signals. Thus, we can choose optical parameters to modulate Ca2+ waves and achieve activation of specific signaling pathways. This novel methodology can be applied to clarify related cell-signaling mechanisms in the future.

SUBMITTER: Lai YS 

PROVIDER: S-EPMC8048425 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Ca<sup>2+</sup> -regulated cell migration revealed by optogenetically engineered Ca<sup>2+</sup> oscillations.

Lai Yi-Shyun YS   Chang Ya-Han YH   Chen Yong-Yi YY   Xu Jixuan J   Yu Chi-Sian CS   Chang Su-Jing SJ   Chen Pai-Sheng PS   Tsai Shaw-Jenq SJ   Chiu Wen-Tai WT  

Journal of cellular physiology 20201126 6


The ability of a single Ca<sup>2+</sup> ion to play an important role in cell biology is highlighted by the need for cells to form Ca<sup>2+</sup> signals in the dimensions of space, time, and amplitude. Thus, spatial and temporal changes in intracellular Ca<sup>2+</sup> concentration are important for determining cell fate. Optogenetic technology has been developed to provide more precise and targeted stimulation of cells. Here, U2OS cells overexpressing Ca<sup>2+</sup> translocating channelrho  ...[more]

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