The mTOR Deficiency in Monocytic Myeloid-Derived Suppressor Cells Protects Mouse Cardiac Allografts by Inducing Allograft Tolerance.

ABSTRACT:

Background

Myeloid-derived suppressor cells (MDSCs) can prevent allograft rejection and induce immune tolerance in transplantation models. Previous studies have demonstrated that inhibition of mTOR signaling can enhance the MDSC protective effect in heart transplantation (HTx) by promoting MDSC expansion. In addition, mTOR inhibition is related to autophagy. The present study investigated the protective mechanism of mTOR-deficient monocytic MDSCs (M-MDSCs) in mouse HTx.

Methods

Myeloid-specific mTOR conditional knockout mice were generated to obtain mTOR^-/- M-MDSCs. The proliferation and immunosuppressive function of mTOR^-/- M-MDSCs were determined by flow cytometry and T cell proliferation assays. The mTOR^-/- M-MDSC intracellular autophagy l

SUBMITTER: Li J 

PROVIDER: S-EPMC8062712 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature