Time-course analysis of Streptococcus sanguinis after manganese depletion reveals changes in glycolytic and nucleic acid metabolites.

ABSTRACT:

Introduction

Manganese is important for the endocarditis pathogen Streptococcus sanguinis. Little is known about why manganese is required for virulence or how it impacts the metabolome of streptococci.

Objectives

We applied untargeted metabolomics to cells and media to understand temporal changes resulting from manganese depletion.

Methods

EDTA was added to a S. sanguinis manganese-transporter mutant in aerobic fermentor conditions. Cell and media samples were collected pre- and post-EDTA treatment. Metabolomics data were generated using positive and negative modes of data acquisition on an LC-MS/MS system. Data were subjected to statistical processing using MetaboAnalyst and time-course analysis using Short Time series Expression Miner (STEM). Recombinant enzymes we

SUBMITTER: Puccio T 

PROVIDER: S-EPMC8064989 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature