Development of an indirect ELISA to specifically detect antibodies against African swine fever virus: bioinformatics approaches.

ABSTRACT:

Background

African swine fever (ASF), characterized by acute, severe, and fast-spreading, is a highly lethal swine infectious disease caused by the African swine fever virus (ASFV), which has caused substantial economic losses to the pig industry worldwide in the past 100 years.

Methods

This study started with bioinformatics methods and verified the epitope fusion protein method's reliability that does not rely on traditional epitope identification. Meanwhile, it will also express and purify the constructed genes through prokaryotic expression and establish antibody detection methods.

Results

The results indicated that the protein had good reactivity and did not cross-react with other swine diseases. The receiver-operating characteristic analysis was performed to veri

SUBMITTER: Gao Z 

PROVIDER: S-EPMC8097255 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature