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Development of an indirect ELISA to specifically detect antibodies against African swine fever virus: bioinformatics approaches.


ABSTRACT:

Background

African swine fever (ASF), characterized by acute, severe, and fast-spreading, is a highly lethal swine infectious disease caused by the African swine fever virus (ASFV), which has caused substantial economic losses to the pig industry worldwide in the past 100 years.

Methods

This study started with bioinformatics methods and verified the epitope fusion protein method's reliability that does not rely on traditional epitope identification. Meanwhile, it will also express and purify the constructed genes through prokaryotic expression and establish antibody detection methods.

Results

The results indicated that the protein had good reactivity and did not cross-react with other swine diseases. The receiver-operating characteristic analysis was performed to verify the determination. The area under the receiver-operating characteristic curve was 0.9991 (95% confidence interval 0.9973 to 1.001).

Conclusions

It was proved that the recombinant protein is feasible as a diagnostic antigen to distinguish ASFV and provides a new idea for ASFV antibody detection.

SUBMITTER: Gao Z 

PROVIDER: S-EPMC8097255 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature

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Development of an indirect ELISA to specifically detect antibodies against African swine fever virus: bioinformatics approaches.

Gao Zhan Z   Shao Jun-Jun JJ   Zhang Guang-Lei GL   Ge Su-Dan SD   Chang Yan-Yan YY   Xiao Lei L   Chang Hui-Yun HY  

Virology journal 20210505 1


<h4>Background</h4>African swine fever (ASF), characterized by acute, severe, and fast-spreading, is a highly lethal swine infectious disease caused by the African swine fever virus (ASFV), which has caused substantial economic losses to the pig industry worldwide in the past 100 years.<h4>Methods</h4>This study started with bioinformatics methods and verified the epitope fusion protein method's reliability that does not rely on traditional epitope identification. Meanwhile, it will also express  ...[more]

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