Ontology highlight
ABSTRACT:
SUBMITTER: Suardiaz R
PROVIDER: S-EPMC8097703 | biostudies-literature | 2021 May
REPOSITORIES: biostudies-literature
Suardíaz Reynier R Lythell Emily E Hinchliffe Philip P van der Kamp Marc M Spencer James J Fey Natalie N Mulholland Adrian J AJ
Organic & biomolecular chemistry 20210501 17
The mcr-1 gene encodes a membrane-bound Zn2+-metalloenzyme, MCR-1, which catalyses phosphoethanolamine transfer onto bacterial lipid A, making bacteria resistant to colistin, a last-resort antibiotic. Mechanistic understanding of this process remains incomplete. Here, we investigate possible catalytic pathways using DFT and ab initio calculations on cluster models and identify a complete two-step reaction mechanism. The first step, formation of a covalent phosphointermediate via transfer of phos ...[more]