Coma-corrected rapid single-particle cryo-EM data collection on the CRYO ARM 300.

ABSTRACT: Single-particle cryogenic electron microscopy has recently become a major method for determining the structures of proteins and protein complexes. This has markedly increased the demand for throughput of high-resolution electron microscopes, which are required to produce high-resolution images at high rates. An increase in data-collection throughput can be achieved by using large beam-image shifts combined with off-axis coma correction, enabling the acquisition of multiple images from a large area of the EM grid without moving the microscope stage. Here, the optical properties of the JEOL CRYO ARM 300 electron microscope equipped with a K3 camera were characterized under off-axis illumination conditions. It is shown that efficient coma correction can be achieved for beam-image shifts with

SUBMITTER: Efremov RG 

PROVIDER: S-EPMC8098478 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature