Single cell RNA-seq analysis of the flexor digitorum brevis mouse myofibers.

ABSTRACT:

Background

Skeletal muscle myofibers can be separated into functionally distinct cell types that differ in gene and protein expression. Current single cell expression data is generally based upon single nucleus RNA, rather than whole myofiber material. We examined if a whole-cell flow sorting approach could be applied to perform single cell RNA-seq (scRNA-seq) in a single muscle type.

Methods

We performed deep, whole cell, scRNA-seq on intact and fragmented skeletal myofibers from the mouse fast-twitch flexor digitorum brevis muscle utilizing a flow-gated method of large cell isolation. We performed deep sequencing of 763 intact and fragmented myofibers.

Results

Quality control metrics across the different gates indicated only 171 of these cells were optimal, with a m

SUBMITTER: Verma RX 

PROVIDER: S-EPMC8127317 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature