Genome-wide associations between alcohol consumption and blood DNA methylation: evidence from twin study.
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ABSTRACT:
SUBMITTER: Lu M
PROVIDER: S-EPMC8147754 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
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Genome-wide associations between alcohol consumption and blood DNA methylation: evidence from twin study.
Lu Meng M Xueying Qin Q Hexiang Peng P Wenjing Gao G Hägg Sara S Weihua Cao C Chunxiao Li L Canqing Yu Y Jun Lv L Zengchang Pang P Liming Cong C Hua Wang W Xianping Wu W Yunzhang Wang W Liming Li L
Epigenomics 20210517 12
<b>Aim:</b> Alcohol intake alters DNA methylation profiles and methylation might mediate the association between alcohol and disease, but limited number of positive CpG sites repeatedly replicated. <b>Materials & methods:</b> In total, 57 monozygotic (MZ) twin pairs discordant for alcohol drinking from the Chinese National Twin Registry and 158 MZ and dizygotic twin pairs in the Swedish Adoption/Twin Study of Aging were evaluated. DNA methylation was detected using the Infinium HumanMethylation4 ...[more]
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Project description:The biological mechanisms driving associations between alcohol consumption and chronic diseases might include epigenetic modification of DNA methylation. We explored the hypothesis that alcohol consumption is associated with methylation in an epigenome-wide association study of blood and normal breast tissue DNA. Infinium HumanMethylation450 BeadChip (Illumina Inc., San Diego, California) array data on blood DNA methylation was examined in a discovery set of 2,878 non-Hispanic white women from the Sister Study (United States, 2004-2015) who provided detailed questionnaire information on lifetime alcohol use. Robust linear regression modeling was used to identify significant associations (false discovery rate of Q < 0.05) between the number of alcoholic drinks per week and DNA methylation at 5,458 cytosine-phosphate-guanine (CpG) sites. Associations were replicated (P < 0.05) for 677 CpGs in an independent set of 187 blood DNA samples from the Sister Study and for 628 CpGs in an independent set of 171 normal breast DNA samples; 1,207 CpGs were replicated in either blood or normal breast, with 98 CpGs replicated in both tissues. Individual gene effects were notable for phosphoglycerate dehydrogenase (PGHDH), peptidyl-prolyl cis-trans isomerase (PPIF), solute carrier 15 (SLC15), solute carrier family 43 member 1 (SLC43A1), and solute carrier family 7 member 11 (SLC7A11). We also found that high alcohol consumption was associated with significantly lower global methylation as measured by the average of CpGs on the entire array.
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Project description: Not available
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