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New Approach for Untangling the Role of Uncommon Calcium-Binding Proteins in the Central Nervous System.

ABSTRACT: Although Ca2+ ion plays an essential role in cellular physiology, calcium-binding proteins (CaBPs) were long used for mainly as immunohistochemical markers of specific cell types in different regions of the central nervous system. They are a heterogeneous and wide-ranging group of proteins. Their function was studied intensively in the last two decades and a tremendous amount of information was gathered about them. Girard et al. compiled a comprehensive list of the gene-expression profiles of the entire EF-hand gene superfamily in the murine brain. We selected from this database those CaBPs which are related to information processing and/or neuronal signalling, have a Ca2+-buffer activity, Ca2+-sensor activity, modulator of Ca2+-channel activity, or a yet unknown function. In this way we created a gene function-based selection of the CaBPs. We cross-referenced these findings with publicly available, high-quality RNA-sequencing and in situ hybridization databases (Human Protein Atlas (HPA), Brain RNA-seq database and Allen Brain Atlas integrated into the HPA) and created gene expression heat maps of the regional and cell type-specific expression levels of the selected CaBPs. This represents a useful tool to predict and investigate different expression patterns and functions of the less-known CaBPs of the central nervous system.

SUBMITTER: Kelemen K

PROVIDER: S-EPMC8156796 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature

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New Approach for Untangling the Role of Uncommon Calcium-Binding Proteins in the Central Nervous System.

Kelemen Krisztina K Szilágyi Tibor T

Brain sciences 20210514 5

Although Ca<sup>2+</sup> ion plays an essential role in cellular physiology, calcium-binding proteins (CaBPs) were long used for mainly as immunohistochemical markers of specific cell types in different regions of the central nervous system. They are a heterogeneous and wide-ranging group of proteins. Their function was studied intensively in the last two decades and a tremendous amount of information was gathered about them. Girard et al. compiled a comprehensive list of the gene-expression pro ...[more]

PMID: 34069107

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New Approach for Untangling the Role of Uncommon Calcium-Binding Proteins in the Central Nervous System.

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New Approach for Untangling the Role of Uncommon Calcium-Binding Proteins in the Central Nervous System.

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