Deciphering transcriptional and functional heterogeneity in hematopoiesis with single-cell genomics.

ABSTRACT:

Purpose of review

Single-cell genomic approaches have uncovered cell fate biases and heterogeneity within hematopoietic subpopulations. However, standard single-cell transcriptomics suffers from high sampling noise, which particularly skews the distribution of lowly expressed genes, such as transcription factors (TFs). This might preclude the identification of rare transcripts that define cell identity and demarcate cell fate biases. Moreover, these studies need to go hand in hand with relevant functional assays to ensure that observed gene expression changes represent biologically meaningful alterations.

Recent findings

Single-cell lineage tracing and functional validation studies have uncovered cell fate bias within transcriptionally distinct hematopoietic stem and progeni

SUBMITTER: Martin-Rufino JD 

PROVIDER: S-EPMC8169609 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature