Myeloid cell-specific Irf5 deficiency stabilizes atherosclerotic plaques in Apoe^-/- mice.

ABSTRACT:

Objective

Interferon regulatory factor (IRF) 5 is a transcription factor known for promoting M1 type macrophage polarization in vitro. Given the central role of inflammatory macrophages in promoting atherosclerotic plaque progression, we hypothesize that myeloid cell-specific deletion of IRF5 is protective against atherosclerosis.

Methods

Female Apoe^-/-Lysm^Cre/+Irf5^fl/fl and Apoe^-/-Irf5^fl/fl mice were fed a high-cholesterol diet for three months. Atherosclerotic plaque size and compositions as well as inflammatory gene expression were analyzed. Mechanistically, IRF5-dependent bone marrow-derived macrophage cytokine profiles were tested under M1 and M2 polarizing conditions. Mixed bone marrow chimeras were generated to de

SUBMITTER: Leipner J 

PROVIDER: S-EPMC8178123 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature