Dataset Information

The therapeutic effects and mechanisms of Long Chai Fang on chronic hepatitis B.

ABSTRACT:

Background

Long Chai Fang (LCF) is a traditional Chinese medicine (TCM) formula for treating chronic hepatitis B (CHB) in clinical settings; however, its related mechanism remains unclear.

Methods

To address this issue, network pharmacology and an integrative method that combines dot-blot hybridization and metabolomics analysis were employed. Network pharmacology was performed to investigate the material basis and potential mechanisms of LCF against CHB. The effect of LCF on Duck hepatitis B virus (DHBV) replication was evaluated. The metabolomics analysis was conducted to identify potential biomarkers in duck serum.

Results

The network pharmacology approach revealed 133 potential active components, 897 drug targets, 979 disease targets, and 185 drug-disease targets, while the Kyoto Encyclopedia of Genes and Genomes enrichment analysis identified 165 pathways. LCF significantly inhibited DHBV-deoxyribonucleic acid replication on day 10 and day 3 after the cessation of treatment. Notably, the low-dose LCF group showed the best inhibitory effect. The obviously sustained anti-DHBV activity of LCF inhibited viral replication, and a rebound reaction was found. Phosphatidylcholine and phosphatidylethanolamine classes, which are mainly involved in liver cell repair and energy metabolism through phospholipid metabolic pathways, were identified by metabolomics analysis.

Conclusions

our results showed that the main active ingredients of LCF appear to be metacarpi, isorhamnetin, glypallichalcone, and phaseolinisoflavan. This study provides novel strategies for using a LCF formula against CHB in future research.

SUBMITTER: Xu T

PROVIDER: S-EPMC8184409 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature

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Publications

The therapeutic effects and mechanisms of Long Chai Fang on chronic hepatitis B.

Xu Tingting T Wang Pei P Zheng Xue X Yan Zhanpeng Z Li Kun K Xu Jindi J Jiang Cuihua C Zhu Fangshi F

Annals of translational medicine 20210501 10

<h4>Background</h4>Long Chai Fang (LCF) is a traditional Chinese medicine (TCM) formula for treating chronic hepatitis B (CHB) in clinical settings; however, its related mechanism remains unclear.<h4>Methods</h4>To address this issue, network pharmacology and an integrative method that combines dot-blot hybridization and metabolomics analysis were employed. Network pharmacology was performed to investigate the material basis and potential mechanisms of LCF against CHB. The effect of LCF on Duck ...[more]

PMID: 34164499

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Dataset Information

The therapeutic effects and mechanisms of Long Chai Fang on chronic hepatitis B.

Background

Methods

Results

Conclusions

Publications

The therapeutic effects and mechanisms of Long Chai Fang on chronic hepatitis B.

Similar Datasets

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