Establishing a Metabolite Extraction Method to Study the Metabolome of Blastocystis Using NMR.

ABSTRACT: Blastocystis is an opportunistic parasite commonly found in the intestines of humans and other animals. Despite its high prevalence, knowledge regarding Blastocystis biology within and outside the host is limited. Analysis of the metabolites produced by this anaerobe could provide insights that can help map its metabolism and determine its role in both health and disease. Due to its controversial pathogenicity, these metabolites could define its deterministic role in microbiome's "health" and/or subsequently resolve Blastocystis' potential impact in gastrointestinal health. A common method for elucidating the presence of these metabolites is through ¹H nuclear magnetic resonance (NMR). However, there are currently no described benchmarked methods available t

SUBMITTER: Newton JM 

PROVIDER: S-EPMC8199492 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature