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Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide.

ABSTRACT: Ca²⁺ imaging is the most frequently used technique to study glial cell physiology. While chemical Ca²⁺ indicators served to visualize and measure changes in glial cell cytosolic Ca²⁺ concentration for several decades, genetically encoded Ca²⁺ indicators (GECIs) have become state of the art in recent years. Great improvements have been made since the development of the first GECI and a large number of GECIs with different physical properties exist, rendering it difficult to select the optimal Ca²⁺ indicator. This review discusses some of the most frequently used GECIs and their suitability for glial cell research.

SUBMITTER: Lohr C

PROVIDER: S-EPMC8226001 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide.

Lohr Christian C Beiersdorfer Antonia A Fischer Timo T Hirnet Daniela D Rotermund Natalie N Sauer Jessica J Schulz Kristina K Gee Christine E CE

Frontiers in cellular neuroscience 20210611

Ca2+ imaging is the most frequently used technique to study glial cell physiology. While chemical Ca2+ indicators served to visualize and measure changes in glial cell cytosolic Ca2+ concentration for several decades, genetically encoded Ca2+ indicators (GECIs) have become state of the art in recent years. Great improvements have been made since the development of the first GECI and a large number of GECIs with different physical properties exist, rend ...[more]

PMID: 34177468

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