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Human Microcephaly Protein RTTN Is Required for Proper Mitotic Progression and Correct Spindle Position.

ABSTRACT: Autosomal recessive primary microcephaly (MCPH) is a complex neurodevelopmental disorder characterized by a small brain size with mild to moderate intellectual disability. We previously demonstrated that human microcephaly RTTN played an important role in regulating centriole duplication during interphase, but the role of RTTN in mitosis is not fully understood. Here, we show that RTTN is required for normal mitotic progression and correct spindle position. The depletion of RTTN induces the dispersion of the pericentriolar protein γ-tubulin and multiple mitotic abnormalities, including monopolar, abnormal bipolar, and multipolar spindles. Importantly, the loss of RTTN altered NuMA/p150Glued congression to the spindle poles, perturbed NuMA cortical localization, and reduced the number and the length of astral microtubules. Together, our results provide a new insight into how RTTN functions in mitosis.

SUBMITTER: Chou EJ

PROVIDER: S-EPMC8229632 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Human Microcephaly Protein RTTN Is Required for Proper Mitotic Progression and Correct Spindle Position.

Chou En-Ju EJ Tang Tang K TK

Cells 20210609 6

Autosomal recessive primary microcephaly (MCPH) is a complex neurodevelopmental disorder characterized by a small brain size with mild to moderate intellectual disability. We previously demonstrated that human microcephaly RTTN played an important role in regulating centriole duplication during interphase, but the role of RTTN in mitosis is not fully understood. Here, we show that RTTN is required for normal mitotic progression and correct spindle position. The deplet ...[more]

PMID: 34207628

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