Transendothelial Electrical Resistance Measurement across the Blood-Brain Barrier: A Critical Review of Methods.

ABSTRACT: The blood-brain barrier (BBB) represents the tightest endothelial barrier within the cardiovascular system characterized by very low ionic permeability. Our aim was to describe the setups, electrodes, and instruments to measure electrical resistance across brain microvessels and culture models of the BBB, as well as critically assess the influence of often neglected physical and technical parameters such as temperature, viscosity, current density generated by different electrode types, surface size, circumference, and porosity of the culture insert membrane. We demonstrate that these physical and technical parameters greatly influence the measurement of transendothelial electrical resistance/resistivity (TEER) across BBB culture models resulting in severalfold differences in TEER values of

SUBMITTER: Vigh JP 

PROVIDER: S-EPMC8231150 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature