Evaluation of Oxford Nanopore MinION RNA-Seq Performance for Human Primary Cells.

ABSTRACT: Transcript sequencing is a crucial tool for gaining a deep understanding of biological processes in diagnostic and clinical medicine. Given their potential to study novel complex eukaryotic transcriptomes, long-read sequencing technologies are able to overcome some limitations of short-read RNA-Seq approaches. Oxford Nanopore Technologies (ONT) offers the ability to generate long-read sequencing data in real time via portable protein nanopore USB devices. This work aimed to provide the user with the number of reads that should be sequenced, through the ONT MinION platform, to reach the desired accuracy level for a human cell RNA study. We sequenced three cDNA libraries prepared from poly-adenosine RNA of human primary cardiac fibroblasts. Since the runs were comparable, they were combined

SUBMITTER: Massaiu I 

PROVIDER: S-EPMC8231517 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature