Dorsal raphe serotonergic neurons promote arousal from isoflurane anesthesia.

ABSTRACT:

Aims

General anesthesia has been widely applied in surgical or nonsurgical medical procedures, but the mechanism behind remains elusive. Because of shared neural circuits of sleep and anesthesia, whether serotonergic system, which is highly implicated in modulation of sleep and wakefulness, regulates general anesthesia as well is worth investigating.

Methods

Immunostaining and fiber photometry were used to assess the neuronal activities. Electroencephalography spectra and burst-suppression ratio (BSR) were used to measure anesthetic depth and loss or recovery of righting reflex to indicate the induction or emergence time of general anesthesia. Regulation of serotonergic system was achieved through optogenetic, chemogenetic, or pharmacological methods.

Results

We found

SUBMITTER: Li A 

PROVIDER: S-EPMC8265942 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature