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Murine cochlear cell sorting and cell-type-specific organoid culture.

ABSTRACT: Neonatal mouse cochlear duct cells can proliferate and grow in vitro into inner ear organoids. Distinctive cochlear duct cell types have different organoid formation capacities. Here, we provide a flow cytometric cell-sorting method that allows the subsequent culture of individual cochlear cell populations. For the efficient culture of the sorted cells, we provide protocols for growing free-floating inner ear organoids, the adherence of organoids to a substrate, and the expansion of organoid-derived inner ear colonies. For complete details on the use and execution of this protocol, please refer to Kubota et al. (2021).

SUBMITTER: Kubota M

PROVIDER: S-EPMC8271165 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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Murine cochlear cell sorting and cell-type-specific organoid culture.

Kubota Marie M Heller Stefan S

STAR protocols 20210707 3

Neonatal mouse cochlear duct cells can proliferate and grow <i>in vitro</i> into inner ear organoids. Distinctive cochlear duct cell types have different organoid formation capacities. Here, we provide a flow cytometric cell-sorting method that allows the subsequent culture of individual cochlear cell populations. For the efficient culture of the sorted cells, we provide protocols for growing free-floating inner ear organoids, the adherence of organoids to a substrate, and the expansion of organ ...[more]

PMID: 34278332

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