Lipids activate skeletal muscle mitochondrial fission and quality control networks to induce insulin resistance in humans.

ABSTRACT:

Background and aims

A diminution in skeletal muscle mitochondrial function due to ectopic lipid accumulation and excess nutrient intake is thought to contribute to insulin resistance and the development of type 2 diabetes. However, the functional integrity of mitochondria in insulin-resistant skeletal muscle remains highly controversial.

Methods

19 healthy adults (age:28.4 ± 1.7 years; BMI:22.7 ± 0.3 kg/m²) received an overnight intravenous infusion of lipid (20% Intralipid) or saline followed by a hyperinsulinemic-euglycemic clamp to assess insulin sensitivity using a randomized crossover design. Skeletal muscle biopsies were obtained after the overnight lipid infusion to evaluate activation of mitochondrial dynamics proteins, ex-vivo mitochondrial membrane poten

SUBMITTER: Axelrod CL 

PROVIDER: S-EPMC8277749 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature