Optimization of Electrical Stimulation for Safe and Effective Guidance of Human Cells.

ABSTRACT: Direct current (DC) electrical stimulation has been shown to have remarkable effects on regulating cell behaviors. Translation of this technology to clinical uses, however, has to overcome several obstacles, including Joule heat production, changes in pH and ion concentration, and electrode products that are detrimental to cells. Application of DC voltages in thick tissues where their thickness is >0.8 mm caused significant changes in temperature, pH, and ion concentrations. In this study, we developed a multifield and -chamber electrotaxis chip, and various stimulation schemes to determine effective and safe stimulation strategies to guide the migration of human vascular endothelial cells. The electrotaxis chip with a chamber thickness of 1 mm allows 10 voltages applied in one experiment.

SUBMITTER: Zhao Z 

PROVIDER: S-EPMC8370347 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature