Dataset Information

Non-enzymatic Transformation of Aflatoxin B₁ by Pseudomonas geniculata m29.

ABSTRACT: Aflatoxin B₁ (AFB₁) is the most harmful mycotoxin produced by filamentous fungi and presents a serious threat to human and animal health. Therefore, it is essential to protect humans and animals from AFB₁-induced acute and chronic toxicity. In this study, Pseudomonas strain m29 having a high efficiency of AFB₁ transformation was isolated from soil. The transformation ratio by m29 was more than 97% within 24 h, and the optimum temperature for transformation was 37°C. Moreover, the AFB₁ transforming activity was mainly attributed to the cell-free supernatant of strain m29. The metabolite that plays a crucial role in AFB₁ transformation is likely 1,2-dimethylhydrazine or 1,1-dimethylhydrazine, as identified by GC-MS and LC-MS analysis. AFB₁ was transformed into a product with molecular formula C₁₇H₁₄O₇. To the best of our knowledge, this is the first study of non-enzymatic AFB₁ transformation by bacteria. Importantly, this AFB₁ transformation mechanism could be universal to various microorganisms.

SUBMITTER: Yao Y

PROVIDER: S-EPMC8383447 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Publications

Non-enzymatic Transformation of Aflatoxin B1 by Pseudomonas geniculata m29.

Yao Yuanyuan Y Shu Xian X Wang Dongdong D Kan Wenjie W Su Pengfei P Hu Hao H Chen Xu X Wang Dacheng D Huang Shengwei S Wu Lifang L

Frontiers in microbiology 20210810

Aflatoxin B1 (AFB1) is the most harmful mycotoxin produced by filamentous fungi and presents a serious threat to human and animal health. Therefore, it is essential to protect humans and animals from AFB1-induced acute and chronic toxicity. In this study, Pseudomonas strain m29 having a high efficiency of AFB1 transformation was isolated from soil. The transformation ratio by m29 was more than 97% within 24 h, and the optimum temperature for tra ...[more]

PMID: 34447365

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Project description:Due to toxicity and persistence of paraquat (a widely used herbicide), eco-friendly remediation approaches to its contamination and effective antidotes to its poisoning have been highly desired and raised increasing concerns. Paraquat degradation was lesser in aerobic soil in comparison with anaerobic soil, and humic-reducing microorganisms (HRMs) play a key role in paraquat anaerobic transformation process. However, the degradation pathways and related mechanisms remain poorly understood. In this study, we investigated the specific interaction mechanisms of the paraquat transformation processes mediated by a humic-reducing strain under anaerobic conditions. A strain of pure culture, designated as PQ01, was successfully isolated from paddy soil using anaerobic enrichment procedure, and identified as Pseudomonas geniculata using phenotypic and phylogenetic analysis. Sucrose, glucose, pyruvate, formic acid, and acetic acid were shown to be favorable electron donors for the reduction of anthrahydroquinone-2,6-disulfonate (AQDS) reduction by PQ01. The strain also had the ability of reducing Fe(III) (hydr)oxides in the presence of sucrose with efficiencies in the order of ferrihydrite > α-FeOOH/γ-FeOOH > γ-Fe2O3 > α-Fe2O3. In the "PQ01 + paraquat + AQDS + sucrose" system, AQDS reduction and paraquat biotransformation by strain PQ01 occurred simultaneously, and the presence of sucrose significantly enhanced the biotransformation. Specific mechanisms of the electron transfer processes are promoted by both PQ01 and AQDS, and proceed in two aspects: (1) paraquat served as electron donor in the anaerobic reduction of AQDS by strain PQ01; (2) AQDS was reduced by PQ01 anaerobic metabolism to produce AH2QDS, which can directly react with paraquat under anaerobic conditions to generate a single crystal compound (molecular formula of the unit structure is C2 6H2 0N2O8S2), causing the paraquat to decline dramatically. In conclusion, this main mechanism included the microbial reduction of AQDS to AH2QDS, followed by the abiotic reaction between AH2QDS and paraquat. This study reported the new characteristics of P. geniculata capable of reducing humics analogs, Fe(III) (hydr)oxides, and paraquat, and proposed a novel electron transformation mechanism of the HRMs' mediated degradation of organic contaminants.

Dataset Information

Non-enzymatic Transformation of Aflatoxin B₁ by Pseudomonas geniculata m29.

Publications

Non-enzymatic Transformation of Aflatoxin B<sub>1</sub> by <i>Pseudomonas geniculata</i> m29.

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