Screening of Neutrophil Activating Factors from a Metagenome Library of Sponge-Associated Bacteria.

ABSTRACT: Marine sponge-associated bacteria are known as bio-active compound produce. We have constructed metagenome libraries of the bacteria and developed a metagenomic screening approach. Activity-based screening successfully identified novel genes and novel enzymes; however, the efficiency was only in 1 out of 10⁴ clones. Therefore, in this study, we thought that bioinformatics could help to reduce screening efforts, and combined activity-based screening with database search. Neutrophils play an important role for the immune system to recognize excreted bacterial by-products as chemotactic factors and are recruited to infection sites to kill pathogens via phagocytosis. These excreted by-products are considered critical triggers that engage the immune system to mount a defense against

SUBMITTER: Okamura Y 

PROVIDER: S-EPMC8402132 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature