Transport engineering for improving the production and secretion of valuable alkaloids in Escherichia coli.

ABSTRACT: Microorganisms can be metabolically engineered to produce specialized plant metabolites. However, these methods are limited by low productivity and intracellular accumulation of metabolites. We sought to use transport engineering for producing reticuline, an important intermediate in the alkaloid biosynthetic pathway. In this study, we established a reticuline-producing Escherichia coli strain into which the multidrug and toxic compound extrusion transporter Arabidopsis AtDTX1 was introduced. AtDTX1 was selected due to its suitable expression in E. coli and its reticuline-transport activity. Expression of AtDTX1 enhanced reticuline production by 11-fold, and the produced reticuline was secreted into the medium. AtDTX1 expression also conferred high plasmid stability an

SUBMITTER: Yamada Y 

PROVIDER: S-EPMC8449128 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature