Nuclei isolation from surgically resected human hippocampus.

ABSTRACT: Single-nucleus RNA sequencing (snRNA-seq), where nuclear transcriptomes are a proxy to cellular transcriptomes, has been used to profile human brain. snRNA-seq is sensitive to tissue processing, tissue quality, postmortem interval time, and cellular debris. This protocol outlines steps for the isolation of high-quality nuclei from surgically resected human brain tissue followed by a sucrose gradient yielding neuronal and non-neuronal nuclei enabling unbiased analysis of various cell types. For complete details on the use and execution of this protocol, please refer to Ayhan et al. (2021).

SUBMITTER: Ayhan F 

PROVIDER: S-EPMC8455478 | biostudies-literature |

REPOSITORIES: biostudies-literature