Vertical sleeve gastrectomy triggers fast β-cell recovery upon overt diabetes.

ABSTRACT:

Objective

The effectiveness of bariatric surgery in restoring β-cell function has been described in type-2 diabetes (T2D) patients and animal models for years, whereas the mechanistic underpinnings are largely unknown. The possibility of vertical sleeve gastrectomy (VSG) to rescue far-progressed, clinically-relevant T2D and to promote β-cell recovery has not been investigated on a single-cell level. Nevertheless, characterization of the heterogeneity and functional states of β-cells after VSG is a fundamental step to understand mechanisms of glycaemic recovery and to ultimately develop alternative, less-invasive therapies.

Methods

We performed VSG in late-stage diabetic db/db mice and analyzed the islet transcriptome using single-cell RNA sequencing (scRNA-seq). Immunohistoc

SUBMITTER: Oppenlander L 

PROVIDER: S-EPMC8487975 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature