Single-cell epigenomics reveals mechanisms of human cortical development.

ABSTRACT: During mammalian development, differences in chromatin state coincide with cellular differentiation and reflect changes in the gene regulatory landscape¹. In the developing brain, cell fate specification and topographic identity are important for defining cell identity² and confer selective vulnerabilities to neurodevelopmental disorders³. Here, to identify cell-type-specific chromatin accessibility patterns in the developing human brain, we used a single-cell assay for transposase accessibility by sequencing (scATAC-seq) in primary tissue samples from the human forebrain. We applied unbiased analyses to identify genomic loci that undergo extensive cell-type- and brain-region-specific changes in accessibility during neurogenesis, and an integrative analysis

SUBMITTER: Ziffra RS 

PROVIDER: S-EPMC8494642 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature