Five-in-One: Simultaneous isolation of multiple major liver cell types from livers of normal and NASH mice.

ABSTRACT: NASH is a chronic liver disease that affects 3%-6% of individuals and requires urgent therapeutic developments. Isolating the key cell types in the liver is a necessary step towards understanding their function and roles in disease pathogenesis. However, traditional isolation methods through gradient centrifugation can only collect one or a few cell types simultaneously and pose technical difficulties when applied to NASH livers. Taking advantage of identified cell surface markers from liver single-cell RNAseq, here we established the combination of gradient centrifugation and antibody-based cell sorting techniques to isolate five key liver cell types (hepatocytes, endothelial cells, stellate cells, macrophages and other immune cells) from a single mouse liver. This method yielded high pur

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC8505823 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature