Systematic reconstruction of an effector-gene network reveals determinants of Salmonella cellular and tissue tropism.
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ABSTRACT:
SUBMITTER: Chen D
PROVIDER: S-EPMC8516738 | biostudies-literature | 2021 Oct
REPOSITORIES: biostudies-literature
Publications
Systematic reconstruction of an effector-gene network reveals determinants of Salmonella cellular and tissue tropism.
Chen Didi D Burford Wesley B WB Pham Giang G Zhang Lishu L Alto Laura T LT Ertelt James M JM Winter Maria G MG Winter Sebastian E SE Way Sing Sing SS Alto Neal M NM
Cell host & microbe 20210917 10
The minimal genetic requirements for microbes to survive within multiorganism communities, including host-pathogen interactions, remain poorly understood. Here, we combined targeted gene mutagenesis with phenotype-guided genetic reassembly to identify a cooperative network of SPI-2 T3SS effector genes that are sufficient for Salmonella Typhimurium (STm) to cause disease in a natural host organism. Five SPI-2 effector genes support pathogen survival within the host cell cytoplasm by coordinating ...[more]
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