Safety and efficacy of human juvenile chondrocyte-derived cell sheets for osteochondral defect treatment.

ABSTRACT: Knee cartilage does not regenerate spontaneously after injury, and a gold standard regenerative treatment algorithm has not been established. This study demonstrates preclinical safety and efficacy of scaffold-free, human juvenile cartilage-derived-chondrocyte (JCC) sheets produced from routine surgical discards using thermo-responsive cultureware. JCCs exhibit stable and high growth potential in vitro over passage 10, supporting possibilities for scale-up to mass production for commercialization. JCC sheets contain highly viable, densely packed cells, show no anchorage-independent cell growth, express mesenchymal surface markers, and lack MHC II expression. In nude rat focal osteochondral defect models, stable neocartilage formation was observed at 4 weeks by JCC sheet transplantation wit

SUBMITTER: Kondo M 

PROVIDER: S-EPMC8520001 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature