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Safety and efficacy of human juvenile chondrocyte-derived cell sheets for osteochondral defect treatment.


ABSTRACT: Knee cartilage does not regenerate spontaneously after injury, and a gold standard regenerative treatment algorithm has not been established. This study demonstrates preclinical safety and efficacy of scaffold-free, human juvenile cartilage-derived-chondrocyte (JCC) sheets produced from routine surgical discards using thermo-responsive cultureware. JCCs exhibit stable and high growth potential in vitro over passage 10, supporting possibilities for scale-up to mass production for commercialization. JCC sheets contain highly viable, densely packed cells, show no anchorage-independent cell growth, express mesenchymal surface markers, and lack MHC II expression. In nude rat focal osteochondral defect models, stable neocartilage formation was observed at 4 weeks by JCC sheet transplantation without abnormal tissue growth over 24 weeks in contrast to the nontreatment group showing no spontaneous cartilage repair. Regenerated cartilage was safranin-O positive, contained type II collagen, aggrecan, and human vimentin, and lacked type I collagen, indicating that the hyaline-like neocartilage formed originates from transplanted JCC sheets rather than host-derived cells. This study demonstrates the safety of JCC sheets and stable hyaline cartilage formation with engineered JCC sheets utilizing a sustainable tissue supply. Cost-benefit and scaling issues for sheet fabrication and use support feasibility of this JCC sheet strategy in clinical cartilage repair.

SUBMITTER: Kondo M 

PROVIDER: S-EPMC8520001 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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Safety and efficacy of human juvenile chondrocyte-derived cell sheets for osteochondral defect treatment.

Kondo Makoto M   Kameishi Sumako S   Kim Kyungsook K   Metzler Nicolas F NF   Maak Travis G TG   Hutchinson Douglas T DT   Wang Angela A AA   Maehara Miki M   Sato Masato M   Grainger David W DW   Okano Teruo T  

NPJ Regenerative medicine 20211015 1


Knee cartilage does not regenerate spontaneously after injury, and a gold standard regenerative treatment algorithm has not been established. This study demonstrates preclinical safety and efficacy of scaffold-free, human juvenile cartilage-derived-chondrocyte (JCC) sheets produced from routine surgical discards using thermo-responsive cultureware. JCCs exhibit stable and high growth potential in vitro over passage 10, supporting possibilities for scale-up to mass production for commercializatio  ...[more]

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2025-06-30 | GSE235008 | GEO