A synthetic promoter system for well-controlled protein expression with different carbon sources in Saccharomyces cerevisiae.

ABSTRACT:

Background

Saccharomyces cerevisiae is an important synthetic biology chassis for microbial production of valuable molecules. Promoter engineering has been frequently applied to generate more synthetic promoters with a variety of defined characteristics in order to achieve a well-regulated genetic network for high production efficiency. Galactose-inducible (GAL) expression systems, composed of GAL promoters and multiple GAL regulators, have been widely used for protein overexpression and pathway construction in S. cerevisiae. However, the function of each element in synthetic promoters and how they interact with GAL regulators are not well known.

Results

Here, a library of synthetic GAL promoters demonstrate that upstream activating sequences (UASs) and core promoters have a

SUBMITTER: Deng J 

PROVIDER: S-EPMC8522093 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature