Bladder tumor ILC1s undergo Th17-like differentiation in human bladder cancer.

ABSTRACT:

Purpose

Human innate lymphoid cells (hILCs) are lineage-negative immune cells that do not express rearranged adaptive antigen receptors. Natural killer (NK) cells are hILCs that contribute to cancer defense. The role of non-NK hILCs in cancer is unclear. Our study aimed to characterize non-NK hILCs in bladder cancer.

Experimental design

Mass cytometry was used to characterize intratumoral non-NK hILCs based on 35 parameters, including receptors, cytokines, and transcription factors from 21 muscle-invasive bladder tumors. Model-based clustering was performed on t-distributed stochastic neighbor embedding (t-SNE) coordinates of hILCs, and the association of hILCs with tumor stage was analyzed.

Results

Most frequent among intratumoral non-NK hILCs were hILC1s, which were

SUBMITTER: Mukherjee N 

PROVIDER: S-EPMC8525153 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature