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Repurposing of Anthocyanin Biosynthesis for Plant Transformation and Genome Editing.

ABSTRACT: CRISPR/Cas9 gene editing technology has been very effective in editing genes in many plant species including rice. Here we further improve the current CRISPR/Cas9 gene editing technology in both efficiency and time needed for isolation of transgene-free and target gene-edited plants. We coupled the CRISPR/Cas9 cassette with a unit that activates anthocyanin biosynthesis, providing a visible marker for detecting the presence of transgenes. The anthocyanin-marker assisted CRISPR (AAC) technology enables us to identify transgenic events even at calli stage, to select transformants with elevated Cas9 expression, and to identify transgene-free plants in the field. We used the AAC technology to edit LAZY1 and G1 and successfully generated many transgene-free and target gene-edited plants at T1 generation. The AAC technology greatly reduced the labor, time, and costs needed for editing target genes in rice.

SUBMITTER: He Y

PROVIDER: S-EPMC8525376 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Repurposing of Anthocyanin Biosynthesis for Plant Transformation and Genome Editing.

He Yubing Y Zhu Min M Wu Junhua J Ouyang Lejun L Wang Rongchen R Sun Hui H Yan Lang L Wang Lihao L Xu Meilian M Zhan Huadong H Zhao Yunde Y

Frontiers in genome editing 20201203

CRISPR/Cas9 gene editing technology has been very effective in editing genes in many plant species including rice. Here we further improve the current CRISPR/Cas9 gene editing technology in both efficiency and time needed for isolation of transgene-free and target gene-edited plants. We coupled the CRISPR/Cas9 cassette with a unit that activates anthocyanin biosynthesis, providing a visible marker for detecting the presence of transgenes. The anthocyanin-marker assisted CRISPR (AAC) technology e ...[more]

PMID: 34713232

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