A High-Throughput Assay for Quantifying Phenotypic Traits of Microalgae.

ABSTRACT: High-throughput methods for phenotyping microalgae are in demand across a variety of research and commercial purposes. Many microalgae can be readily cultivated in multi-well plates for experimental studies which can reduce overall costs, while measuring traits from low volume samples can reduce handling. Here we develop a high-throughput quantitative phenotypic assay (QPA) that can be used to phenotype microalgae grown in multi-well plates. The QPA integrates 10 low-volume, relatively high-throughput trait measurements (growth rate, cell size, granularity, chlorophyll a, neutral lipid content, silicification, reactive oxygen species accumulation, and photophysiology parameters: ETRmax, I_k, and alpha) into one workflow. We demonstrate the utility of the QPA on Thalassiosira</

SUBMITTER: Argyle PA 

PROVIDER: S-EPMC8528002 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature