Dataset Information

Substrate control of sulphur utilisation and microbial stoichiometry in soil: Results of ¹³C, ¹⁵N, ¹⁴C, and ³⁵S quad labelling.

ABSTRACT: Global plant sulphur (S) deficiency is increasing because of a reduction in sulphate-based fertiliser application combined with continuous S withdrawal during harvest. Here, we applied ¹³C, ¹⁵N, ¹⁴C, and ³⁵S quad labelling of the S-containing amino acids cysteine (Cys) and methionine (Met) to understand S cycling and microbial S transformations in the soil. The soil microorganisms absorbed the applied Cys and Met within minutes and released SO₄^2- within hours. The SO₄^2- was reutilised by the MB within days. The initial microbial utilisation and SO₄^2- release were determined by amino acid structure. Met released 2.5-fold less SO₄^2- than Cys. The microbial biomass retained comparatively more C and S from Met than Cys. The microorganisms decomposed Cys to pyruvate and H₂S whereas they converted Met to α-ketobutyrate and S-CH₃. The microbial stoichiometries of C, N, and S derived from Cys and Met were balanced after 4 d by Cys-derived SO₄^2- uptake and Met-derived CO₂ release. The microbial C:N:S ratio dynamics showed rapid C utilisation and loss, stable N levels, and S accumulation. Thus, short-term organic S utilisation by soil microorganisms is determined by amino acid structure whilst long-term organic S utilisation by soil microorganisms is determined by microbially controlled stoichiometry.

SUBMITTER: Ma Q

PROVIDER: S-EPMC8528905 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature

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Publications

Substrate control of sulphur utilisation and microbial stoichiometry in soil: Results of 13C, 15N, 14C, and 35S quad labelling.

Ma Qingxu Q Kuzyakov Yakov Y Pan Wankun W Tang Sheng S Chadwick David R DR Wen Yuan Y Hill Paul W PW Macdonald Andy A Ge Tida T Si Linlin L Wu Lianghuan L Jones Davey L DL

The ISME journal 20210511 11

Global plant sulphur (S) deficiency is increasing because of a reduction in sulphate-based fertiliser application combined with continuous S withdrawal during harvest. Here, we applied 13C, 15N, 14C, and 35S quad labelling of the S-containing amino acids cysteine (Cys) and methionine (Met) to understand S cycling and microbial S transformations in the soil. The soil microorganisms absorbed the applied Cys and Met within minutes and released SO4</s ...[more]

PMID: 33976391

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Project description:Determining the accurate chemical structures of synthesized compounds is essential for biomedical studies and computer-assisted drug design. The unequivocal determination of N-adamantylation or N-arylation site(s) in nitrogen-rich heterocycles, characterized by a low density of hydrogen atoms, using NMR methods at natural isotopic abundance is difficult. In these compounds, the heterocyclic moiety is covalently attached to the carbon atom of the substituent group that has no bound hydrogen atoms, and the connection between the two moieties of the compound cannot always be established via conventional 1H-1H and 1H-13C NMR correlation experiments (COSY and HMBC, respectively) or nuclear Overhauser effect spectroscopy (NOESY or ROESY). The selective incorporation of 15N-labelled atoms in different positions of the heterocyclic core allowed for the use of 1H-15N (JHN) and 13C-15N (JCN) coupling constants for the structure determinations of N-alkylated nitrogen-containing heterocycles in solution. This method was tested on the N-adamantylated products in a series of azolo-1,2,4-triazines and 1,2,4-triazolo[1,5-a]pyrimidine. The syntheses of adamantylated azolo-azines were based on the interactions of azolo-azines and 1-adamatanol in TFA solution. For azolo-1,2,4-triazinones, the formation of mixtures of N-adamantyl derivatives was observed. The JHN and JCN values were measured using amplitude-modulated 1D 1H spin-echo experiments with the selective inversion of the 15N nuclei and line-shape analysis in the 1D 13С spectra acquired with selective 15N decoupling, respectively. Additional spin-spin interactions were detected in the 15N-HMBC spectra. NMR data and DFT (density functional theory) calculations permitted to suggest a possible mechanism of isomerization for the adamantylated products of the azolo-1,2,4-triazines. The combined analysis of the JHN and JCN couplings in 15N-labelled compounds provides an efficient method for the structure determination of N-alkylated azolo-azines even in the case of isomer formation. The isomerization of adamantylated tetrazolo[1,5-b][1,2,4]triazin-7-ones in acidic conditions occurs through the formation of the adamantyl cation.

Dataset Information

Substrate control of sulphur utilisation and microbial stoichiometry in soil: Results of ¹³C, ¹⁵N, ¹⁴C, and ³⁵S quad labelling.

Publications

Substrate control of sulphur utilisation and microbial stoichiometry in soil: Results of <sup>13</sup>C, <sup>15</sup>N, <sup>14</sup>C, and <sup>35</sup>S quad labelling.

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Dataset Information

Substrate control of sulphur utilisation and microbial stoichiometry in soil: Results of 13C, 15N, 14C, and 35S quad labelling.

Publications

Substrate control of sulphur utilisation and microbial stoichiometry in soil: Results of &lt;sup&gt;13&lt;/sup&gt;C, &lt;sup&gt;15&lt;/sup&gt;N, &lt;sup&gt;14&lt;/sup&gt;C, and &lt;sup&gt;35&lt;/sup&gt;S quad labelling.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Substrate control of sulphur utilisation and microbial stoichiometry in soil: Results of ¹³C, ¹⁵N, ¹⁴C, and ³⁵S quad labelling.

Substrate control of sulphur utilisation and microbial stoichiometry in soil: Results of <sup>13</sup>C, <sup>15</sup>N, <sup>14</sup>C, and <sup>35</sup>S quad labelling.