Dataset Information

Enlisting wild grass genes to combat nitrification in wheat farming: A nature-based solution.

ABSTRACT: Active nitrifiers and rapid nitrification are major contributing factors to nitrogen losses in global wheat production. Suppressing nitrifier activity is an effective strategy to limit N losses from agriculture. Production and release of nitrification inhibitors from plant roots is termed "biological nitrification inhibition" (BNI). Here, we report the discovery of a chromosome region that controls BNI production in "wheat grass" Leymus racemosus (Lam.) Tzvelev, located on the short arm of the "Lr#3Nsb" (Lr#n), which can be transferred to wheat as T3BL.3NsbS (denoted Lr#n-SA), where 3BS arm of chromosome 3B of wheat was replaced by 3NsbS of L. racemosus We successfully introduced T3BL.3NsbS into the wheat cultivar "Chinese Spring" (CS-Lr#n-SA, referred to as "BNI-CS"), which resulted in the doubling of its BNI capacity. T3BL.3NsbS from BNI-CS was then transferred to several elite high-yielding hexaploid wheat cultivars, leading to near doubling of BNI production in "BNI-MUNAL" and "BNI-ROELFS." Laboratory incubation studies with root-zone soil from field-grown BNI-MUNAL confirmed BNI trait expression, evident from suppression of soil nitrifier activity, reduced nitrification potential, and N2O emissions. Changes in N metabolism included reductions in both leaf nitrate, nitrate reductase activity, and enhanced glutamine synthetase activity, indicating a shift toward ammonium nutrition. Nitrogen uptake from soil organic matter mineralization improved under low N conditions. Biomass production, grain yields, and N uptake were significantly higher in BNI-MUNAL across N treatments. Grain protein levels and breadmaking attributes were not negatively impacted. Wide use of BNI functions in wheat breeding may combat nitrification in high N input-intensive farming but also can improve adaptation to low N input marginal areas.

SUBMITTER: Subbarao GV

PROVIDER: S-EPMC8536370 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature

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Enlisting wild grass genes to combat nitrification in wheat farming: A nature-based solution.

Subbarao Guntur V GV Kishii Masahiro M Bozal-Leorri Adrian A Ortiz-Monasterio Ivan I Gao Xiang X Ibba Maria Itria MI Karwat Hannes H Gonzalez-Moro M B MB Gonzalez-Murua Carmen C Yoshihashi Tadashi T Tobita Satoshi S Kommerell Victor V Braun Hans-Joachim HJ Iwanaga Masa M

Proceedings of the National Academy of Sciences of the United States of America 20210801 35

Active nitrifiers and rapid nitrification are major contributing factors to nitrogen losses in global wheat production. Suppressing nitrifier activity is an effective strategy to limit <i>N</i> losses from agriculture. Production and release of nitrification inhibitors from plant roots is termed "biological nitrification inhibition" (BNI). Here, we report the discovery of a chromosome region that controls BNI production in "wheat grass" <i>Leymus racemosus</i> (Lam.) Tzvelev, located on the shor ...[more]

PMID: 34426500

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Dataset Information

Enlisting wild grass genes to combat nitrification in wheat farming: A nature-based solution.

Publications

Enlisting wild grass genes to combat nitrification in wheat farming: A nature-based solution.

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