Spatially-resolved nanometer-scale measurement of cartilage extracellular matrix mobility.

ABSTRACT:

Objective

Tissues have complex structures, comprised of solid and fluid phases. Improved understanding of interactions between joint fluid and extracellular matrix (ECM) is required in models of cartilage mechanics. X-ray photon correlation spectroscopy (XPCS) directly measures nanometer-scale dynamics and can provide insight into biofluid-biosolid interactions in cartilage. This study applies XPCS to evaluate dynamic interactions between intact cartilage and biofluids.

Design

Cartilage biopsies were collected from bovine femoral condyles. During XPCS measurements, cartilage samples were exposed to different fluids: deionized water, PBS, synovial fluid, or sonicated synovial fluid. ECM-biofluid interactions were also assessed at different length scales and different depths f

SUBMITTER: Partain BD 

PROVIDER: S-EPMC8543368 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature