High-yield 'one-pot' biosynthesis of raspberry ketone, a high-value fine chemical.

ABSTRACT: Cell-free extract and purified enzyme-based systems provide an attractive solution to study biosynthetic strategies towards a range of chemicals. 4-(4-hydroxyphenyl)-butan-2-one, also known as raspberry ketone, is the major fragrance component of raspberry fruit and is used as a natural additive in the food and sports industry. Current industrial processing of the natural form of raspberry ketone involves chemical extraction from a yield of ∼1-4 mg kg^-1 of fruit. Due to toxicity, microbial production provides only low yields of up to 5-100 mg L^-1. Herein, we report an efficient cell-free strategy to probe into a synthetic enzyme pathway that converts either L-tyrosine or the precursor, 4-(4-hydroxyphenyl)-buten-2-one, into raspberry ketone at up to 100% conversion. As

SUBMITTER: Moore SJ 

PROVIDER: S-EPMC8546603 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature