Dataset Information

Low-Intensity Exercise Modulates Gut Microbiota to Fight Against Radiation-Induced Gut Toxicity in Mouse Models.

ABSTRACT: Radiation-induced gastrointestinal (GI) tract toxicity halts radiotherapy and degrades the prognosis of cancer patients. Physical activity defined as "any bodily movement produced by skeletal muscle that requires energy expenditure" is a beneficial lifestyle modification for health. Here, we investigate whether walking, a low-intensity form of exercise, could alleviate intestinal radiation injury. Short-term (15 days) walking protected against radiation-induced GI tract toxicity in both male and female mice, as judged by longer colons, denser intestinal villi, more goblet cells, and lower expression of inflammation-related genes in the small intestines. High-throughput sequencing and untargeted metabolomics analysis showed that walking restructured the gut microbiota configuration, such as elevated Akkermansia muciniphila, and reprogramed the gut metabolome of irradiated mice. Deletion of gut flora erased the radioprotection of walking, and the abdomen local irradiated recipients who received fecal microbiome from donors with walking treatment exhibited milder intestinal toxicity. Oral gavage of A. muciniphila mitigated the radiation-induced GI tract injury. Importantly, walking did not change the tumor growth after radiotherapy. Together, our findings provide novel insights into walking and underpin that walking is a safe and effective form to protect against GI syndrome of patients with radiotherapy without financial burden in a preclinical setting.

SUBMITTER: Wang B

PROVIDER: S-EPMC8566984 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Low-Intensity Exercise Modulates Gut Microbiota to Fight Against Radiation-Induced Gut Toxicity in Mouse Models.

Wang Bin B Jin Yu-Xiao YX Dong Jia-Li JL Xiao Hui-Wen HW Zhang Shu-Qin SQ Li Yuan Y Chen Zhi-Yuan ZY Yang Xiao-Dong XD Fan Sai-Jun SJ Cui Ming M

Frontiers in cell and developmental biology 20211021

Radiation-induced gastrointestinal (GI) tract toxicity halts radiotherapy and degrades the prognosis of cancer patients. Physical activity defined as "any bodily movement produced by skeletal muscle that requires energy expenditure" is a beneficial lifestyle modification for health. Here, we investigate whether walking, a low-intensity form of exercise, could alleviate intestinal radiation injury. Short-term (15 days) walking protected against radiation-induced GI tract toxicity in both male and ...[more]

PMID: 34746120

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Project description:INTRODUCTION:Intestinal metabolism and microbiota profiles are impaired in obesity and insulin resistance. Moreover, dysbiotic gut microbiota has been suggested to promote systemic low-grade inflammation and insulin resistance through the release of endotoxins particularly lipopolysaccharides. We have previously shown that exercise training improves intestinal metabolism in healthy men. To understand whether changes in intestinal metabolism interact with gut microbiota and its release of inflammatory markers, we studied the effects of sprint interval (SIT) and moderate-intensity continuous training (MICT) on intestinal metabolism and microbiota in subjects with insulin resistance. METHODS:Twenty-six, sedentary subjects (prediabetic, n = 9; type 2 diabetes, n = 17; age, 49 [SD, 4] yr; body mass index, 30.5 [SD, 3]) were randomized into SIT or MICT. Intestinal insulin-stimulated glucose uptake (GU) and fatty acid uptake (FAU) from circulation were measured using positron emission tomography. Gut microbiota composition was analyzed by 16S rRNA gene sequencing and serum inflammatory markers with multiplex assays and enzyme-linked immunoassay kit. RESULTS:V?O2peak improved only after SIT (P = 0.01). Both training modes reduced systematic and intestinal inflammatory markers (tumor necrosis factor-?, lipopolysaccharide binding protein) (time P < 0.05). Training modified microbiota profile by increasing Bacteroidetes phylum (time P = 0.03) and decreasing Firmicutes/Bacteroidetes ratio (time P = 0.04). Moreover, there was a decrease in Clostridium genus (time P = 0.04) and Blautia (time P = 0.051). Only MICT decreased jejunal FAU (P = 0.02). Training had no significant effect on intestinal GU. Colonic GU associated positively with Bacteroidetes and inversely with Firmicutes phylum, ratio Firmicutes/Bacteroidetes and Blautia genus. CONCLUSIONS:Intestinal substrate uptake associates with gut microbiota composition and whole-body insulin sensitivity. Exercise training improves gut microbiota profiles and reduces endotoxemia.

Project description:BackgroundSpirulina, which are filamentous cyanobacteria, have gained significant popularity in the food industry, medicine, and aquaculture.MethodsIn this study, our objective was to explore the influence of Spirulina on the gut microbiota and exercise capacity of mice undergoing high-intensity exercise. Twenty-four male BALB/c mice were divided into four groups, with six mice in each group. These groups included the control group (Control, in which the mice received saline gavage and were permitted free movement), the exercise group (Running, in which the mice were gavaged with the same volume of saline and subjected to a structured exercise regimen), and the Spirulina intervention groups (including SpirulinaLow and SpirulinaHigh). In the Spirulina intervention groups, the mice were orally administered with Spirulina at doses of 100 and 300 mg/kg/day for four weeks while simultaneously participating in the exercise protocol.ResultsThe results illustrated that the Running group mice subjected to intense exercise exhibited reduced weight and tension, acute damage to muscle and liver tissues, oxidative stress, and an imbalance in the gut microbiota compared with that of the Control group. However, high-concentration Spirulina supplementation was found to increase the tensile strength of the exercise mice by 1.27 ± 0.19 fold (p < 0.05) and ameliorate muscle and liver damage. In the SpirulinaHigh group, the levels of certain indicators related to muscle oxidative stress, including reactive oxygen species, total superoxide dismutase, and catalase, were decreased by 39 ± 5.32% (p < 0.01) and increased by 1.11 ± 0.17 fold and 1.19 ± 0.22 fold (p < 0.01) compared to the Running group. Additionally, a correlation analysis reveals that the alterations in gut microbiota induced by Spirulina might be associated with the indicators of tension and oxidative stress.ConclusionsCollectively, these findings point to the fact that Spirulina can effectively mitigate the acute damage to muscles and the liver induced by intense exercise in mice by enhancing antioxidant capacity and regulating the gut microbiota, thereby providing novel insights into the mechanism underlying the enhancement of exercise function.

Dataset Information

Low-Intensity Exercise Modulates Gut Microbiota to Fight Against Radiation-Induced Gut Toxicity in Mouse Models.

Publications

Low-Intensity Exercise Modulates Gut Microbiota to Fight Against Radiation-Induced Gut Toxicity in Mouse Models.

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